Part:BBa_K1104201
TrxC promoter
Introduction
TrxC promoter is controlled by OxyR (transcription factor), which is activated by ROS(Reactive Oxygen Species). An intramolecular disulfide bond between Cys199 and Cys208 will form in an OxyR transcription factor, and active OxyR molecule will bind to the binding site of TrxCp, making the following circuit turned on.
In our project: Bee. coli, we use TrxCp as the sensor of Bee. coli to sense Nosema ceranae. After Nosema ceranae enter bees' midgut, the natural immune reaction will lead to ROS released by epithelia of bees' midgut. We select several promoters that can be induced by OxyR(Part:BBa_K1104200), which is activated by ROS. One of these promoters is TrxCp.
Information of TrxCp on [http://www.ecocyc.org/ECOLI/NEW-IMAGE?type=OPERON&object=TU0-3104 Ecocyc]
We get information of TrxCp from [http://www.ecocyc.org/ECOLI/NEW-IMAGE?type=OPERON&object=TU0-3104 Ecocyc],
TrxCp is regulated by σ70, and there are two TFBSs upstream for OxyR. We also did some literature study, according to the citation.
In E. coli genome, thioredoxin(trxC) will express in response to elevated H2O2 level. Expression of genes encoding thioredoxin(trxC) is regulated by OxyR. Oxidized OxyR binds directly to TrxCp and induce its expression.
 OxyR TFBS1 Sequence of TrxCp(taken from [http://www.ecocyc.org/ECOLI/NEW-IMAGE?type=OPERON&object=TU0-3104 Ecocyc]) |
 OxyR TFBS2 Sequence of TrxCp(taken from [http://www.ecocyc.org/ECOLI/NEW-IMAGE?type=OPERON&object=TU0-3104 Ecocyc]) |
- Reference: Ritz, D. (January 28, 2000). Thioredoxin 2 Is Involved in the Oxidative Stress Response in Escherichia coli. Journal of Biological Chemistry, 275, 4, 2505-2512.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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