Generator

Part:BBa_K1151006

Designed by: Emanuele Rizzo   Group: iGEM13_UniSalento_Lecce   (2013-09-21)
Revision as of 10:15, 1 October 2013 by LeleBiotec (Talk | contribs)

HpNikR coding device

It's a simple coding device of HpNikR, obtained as ligation product of BBa_J04500 + BBa_K1151000 + BBa_B0015. The protein is synthesized when IPTG is present.

Protein expression

The plasmid containing this part has been inserted inside of BL21 cells, and we proceeded with the classic protocol of induction with IPTG for a time of 1, 2 and 4 hours. 

                                             Rit.jpg

Figure 1: Induction results.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aHpNikR coding device