Coding

Part:BBa_K1111006:Design

Designed by: Luisa Spisak, Marcelle Isaline Arrigo   Group: iGEM13_EPF_Lausanne   (2013-09-19)
Revision as of 20:42, 30 September 2013 by Miarrigo (Talk | contribs) (→‎Source)


Matrix metalloprotease 2 (MMP2)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 429
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to activate the enzyme a special buffer is needed. We also added a his tag to the protein to purify it.


Source

Enterococcus faecalis, Gram positive bacterium

References