Reporter

Part:BBa_K1041003

Designed by: Lucy Clark   Group: iGEM13_NRP-UEA-Norwich   (2013-09-19)
Revision as of 13:49, 30 September 2013 by Shaimaa93 (Talk | contribs)

Neomycin Resistance Reporter Gene

Team NRP-UEA_Norwich 2013 desgined this part using biobricks BBa_K1041000 and BBa_K1041002. These biobricks both contain a Nde1 site after their promoter sequence, enabling a restriction digest to be performed. The Neomycin resistance coding gene was excised from BBa_K1041002 and ligated in front of the promoter of BBa_K1041000 to create a new biobrick.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 855
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 704
    Illegal SapI.rc site found at 914


[edit]
Categories
Parameters
None