Part:BBa_K1111004:Experience

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Applications of BBa_K1111004

We wanted to test if the promoter that we cloned into the plasmid really can be induced upon external acidification. In order to do that, we transformed bacteria with it, incubated them at different pH and then measured their GFP expression using a plate reader.

Sequencing

The sequencing result showed that there were no mutations in the insert

Figure 1: Sequencing result of the Cad promoter using one foreward Primer that binds directly to the beginning of the promoter

User Reviews

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