Composite

Part:BBa_K1111004:Design

Designed by: Luisa Spisak   Group: iGEM13_EPF_Lausanne   (2013-09-19)
Revision as of 08:58, 30 September 2013 by GigiGoesGugu (Talk | contribs) (→‎Source)


superfolded GFP driven by CAD promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 496
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 474
    Illegal SapI.rc site found at 540


Design Notes

We assembled the promter into an already existing Plasmid containing the superfoded GFP.


Source

Escherichia Coli, K-12, Substrain MG1655

References

Journal of Bacteriology
Nucleotode Sequence of the Escherichiea coli cad Operon: a system for neutralization of Low Extracellular pH
Shi-Yuan meng and George N. Bennett
J. Bacteriol 1992

Journal of Bacteriology
Regulation of the Escherichia coli cad Operon: Location of a Site Required for Acid Induction
Shi-Yuan meng and George N. Bennett
J. Bacteriol 1992