Composite

Part:BBa_K1111004:Design

Designed by: Luisa Spisak   Group: iGEM13_EPF_Lausanne   (2013-09-19)
Revision as of 08:57, 30 September 2013 by GigiGoesGugu (Talk | contribs) (→‎References)


superfolded GFP driven by CAD promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 496
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 474
    Illegal SapI.rc site found at 540


Design Notes

We assembled the promter into an already existing Plasmid containing the superfoded GFP.


Source

e.Coli MG1655

References

Journal of Bacteriology
Nucleotode Sequence of the Escherichiea coli cad Operon: a system for neutralization of Low Extracellular pH
Shi-Yuan meng and George N. Bennett
J. Bacteriol 1992

Journal of Bacteriology
Regulation of the Escherichia coli cad Operon: Location of a Site Required for Acid Induction
Shi-Yuan meng and George N. Bennett
J. Bacteriol 1992