Part:BBa_K1150031

uniCAS UV Light Switch Part II - Repressor

uniCAS UV Light Switch Part II - Repressor
Function	Repression domain of UVB light induced gene expression control
Use in	Mammalians
RFC standard	RFC 25
Backbone	pSB1C3
Submitted by	[http://2013.igem.org/Team:Freiburg Freiburg 2013]

The fusion protein COP1-KRAB is an interaction partner of dCas9-UVR8. When crRNA and tracrRNA bind to Cas9, the protein is able to bind complementary DNA. If the system is exposed to UVB light (311 nm), the UVR8 receptor changes its configuration and recruits this COP1-KRAB protein. This represses gene expression induced by UVB light.

Biology and Usage

E3-ubiquitin ligase CONSTITUTIVELY PHOTOMORPHOGENIC 1 (COP1) is the interaction partner of ultraviolet B (UVB) light receptor UVR8 [1]. When UVB light is perceived by the UVR8 photoreceptor it monomerizes and therefore changes in its open confuguration. Then it recruits COP1.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 576
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1373
  Illegal BsaI.rc site found at 1450
  Illegal BsaI.rc site found at 1876
  Illegal SapI.rc site found at 962
  Illegal SapI.rc site found at 1840