![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K1189031
Designed by: Ali Honarmand, Somshukla Chaudhuri Group: iGEM13_Calgary (2013-09-17)
TALE-A linked to beta-lactamase with a his tag under a lacI promoter
This part was built to function as both our detector (TALE A) and our reporter (beta-lactamase). The part was built with the lacI IPTG inducible promoter J04500, with RBS, and it has a His-tag for protein purification.
TALE A was inspired by the award winning TALE A from the award winning 2012 Slovenian iGEM project. The iGEM Calgary 2013 used this TALE and its associated DNA binding sequence to build a proof of concept TALE based DNA detector. In the case of BBa_K1189031, the Calgary team used beta-lactamase as a reporter enzyme to indicate when the TALE is bound to DNA.![](https://static.igem.org/mediawiki/parts/5/51/YYC_2013_PlacI_%2B_His_%2B_TALE_%2B_Blac.jpg)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1922
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2651
Illegal AgeI site found at 677
Illegal AgeI site found at 1258 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |