Project

Part:BBa_K1223002:Design

Designed by: Orr Schlesinger   Group: iGEM13_BGU_Israel   (2013-09-07)
Revision as of 15:47, 29 September 2013 by Schlez (Talk | contribs) (→‎Source)


P.A.S.E. 2 cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1025
    Illegal BamHI site found at 1193
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 361
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 301
    Illegal SapI site found at 511


Design Notes

selection for cassette integration into E.coli BL21 genome


Source

this construct was synthesized in one piece. the sources of the sequences used in this part are genbank (TyrS CDS, KanR) and the iGEM catalog (BBa_k354000,BBa_b0034).

References