Regulatory

Part:BBa_K1073005

Designed by: iGEM Team Braunschweig 2013   Group: iGEM13_Braunschweig   (2013-09-16)
Revision as of 15:47, 29 September 2013 by JonasZ (Talk | contribs) (→‎Usage and Biology)

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Constitutive promoter (medium to strong) and RBS

This part combines a constitutive promoter with an RBS and can be cloned in front of a gene of interest. It is a combination of BBa_J23106 and BBa_B0032.

Usage and Biology

This construct can be cloned in fron of a gene of interest to tune a medium to strong transcription level of constitutivly expressed genes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None