Translational_Unit

Part:BBa_K1065103:Design

Designed by: Caterina Marchioretti Michele Pedrotti   Group: iGEM13_UNITN-Trento   (2013-08-28)
Revision as of 12:14, 29 September 2013 by Cate (Talk | contribs) (→‎References)

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PchBA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 326
    Illegal NgoMIV site found at 730
    Illegal NgoMIV site found at 855
    Illegal NgoMIV site found at 866
    Illegal NgoMIV site found at 1147
    Illegal NgoMIV site found at 1615
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was built by starting from the extraction of BBa_J45319 from the iGEM Registry 2012 Kit Distribution, then it was subcloned in pSB1C3 by standard assembly

Source

PchBA genes were extracted from Pseudomonas aeruginosa.

References

1. Laura Serino at al., (1995). Structural genes for salicylate biosynthesis from chorismate in Pseudomonas aeruginosa, Molecular and General Genetics MGG, Volume 249, Issue 2, pp 217-228.