Part:BBa_K1073032
Autoinducer synthase LasI + LVA with combined eforRed chromoprotein expression cassette
This part builds the basis for the construction of an expression cassette of the autoinducer synthase LasI which synthesizes N-3-oxododecanoyl-homoserine lactone. It is combined with a expression cassette of the chromoprotein eforRed. The device lacks a promoter in front of lasI and therefore only leads to expression of eforRed.
Usage and Biology
This device can be used to produce the autoinducer N-3-oxododecanoyl-HSL of the las qourum sensing system of Pseudomonas aeruginosa. N-3-oxododecanoyl-HSL builds a complex with the transcription regulator LasR. This complex then binds to specific sequences in the las promoter region activating the expression of genes located downstream (not included in this device). Since this contruct does not include a promoter in front of lasI it can be combined with various regulatory regions (e.g. a constitutively or regulated promoter) depending on intended use.
The constitutively expressed chromoprotein eforRed is added to the construct and can be used as a reporter. EforRed exhibits a pink color when expressed and can be detected in less than 24 h during cultivation on agar plates or in liquid culture (see BBa_K592012).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 838
Illegal NheI site found at 861 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 680
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 242
- 1000COMPATIBLE WITH RFC[1000]
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