Part:BBa_K1072010

Odr-10(diacetyl)GPCR with optimized codon

Odr-10(diacetyl)GPCR encodes a diacetyl-sensing G-protein coupled receptor, originating from the C. elegans.

If BBa_K1072010 was transformed into the yeast, the pheromone receptors will be actived and coupled to a heterotrimeric G protein. pathway is composed of Gpa1 (Gα), Ste4 (Gβ) and Ste18 (Gγ) subunits.The Gβγ particle transmits the signal to a MAP kinase module and finally follow a inducible promotor FUS1, which will links a reproter, such as GFP, His3 or LacZ.

Figure 1. a diagram of Odr-10, 7-transmembrane receptors.

We fuse it with plasma membrane protein,Odr-10 and expressed in yeast successfully figure 1.

Figure 1. fluorescence was detected around plasma membrane

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]