Composite

Part:BBa_J37027:Design

Designed by: John Chattaway   Group: iGEM06_Imperial   (2006-08-01)
Revision as of 15:36, 1 August 2006 by Jonny (Talk | contribs)

Pops Blocker (Cre/Lox system)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 340
    Illegal AgeI site found at 452
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Used specific lox sites to make this irreversable


Source

From Registry: BBa_B0015 BBa_I13521

Lox Sites: Zuwen Zhang and Beat Lutz (2002) Cre Recombinase-mediated inversion using lox66 and lox71: method to introduce conditional point mutations into the CREB-binding protein NUCLEAIC ACIDS RESEARCH

References