Coding

Part:BBa_K1033104:Experience

Designed by: Magnus Bergman, Pontus Danielsson, Nils Anlind, Alexander Wirtanen, Mailin Borg, Thorsteinn Olaufsson, Niclas Sandberg, Christoffer Frisk   Group: iGEM13_Uppsala   (2013-09-04)
Revision as of 13:15, 28 September 2013 by Magnus (Talk | contribs) (Applications of BBa_K1033104)

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Applications of BBa_K1033104

This part was sucessfully cloned from the chromosome of a d5alpha e.coli with overhangs containing ribosome binding site B0034 and biobrick restriction sites. As it contained an illegal SpeI site, mutagenisation was performed, with sucess. The sequencencing was done at GATC biotech and Uppsla Genome center. We were unsucessful in assembling any promoter to the mutagenized construct, and as such could not further characterize the part.

User Reviews

UNIQ5ae9cb5c05a5d121-partinfo-00000000-QINU UNIQ5ae9cb5c05a5d121-partinfo-00000001-QINU