Coding

Part:BBa_K1033104:Experience

Designed by: Magnus Bergman, Pontus Danielsson, Nils Anlind, Alexander Wirtanen, Mailin Borg, Thorsteinn Olaufsson, Niclas Sandberg, Christoffer Frisk   Group: iGEM13_Uppsala   (2013-09-04)
Revision as of 13:13, 28 September 2013 by Magnus (Talk | contribs) (Applications of BBa_K1033104)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1033104

This part was sucessfully cloned from the chromosome of a d5alpha e.coli with overhangs containing ribosome binding site B0034. As it contained an ilegal SpeI site, mutagenisation was sucessfully performed. The sequencencing was done at GATC biotech and Uppsla Genome center. We were unsucessful in assembling any promoter to the mutagenized construct, and as such could not further characterize the part.

User Reviews

UNIQe3f54b3b7fb3a690-partinfo-00000000-QINU UNIQe3f54b3b7fb3a690-partinfo-00000001-QINU