Part:BBa_K1073022:Experience

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Applications of BBa_K1073022

When eforRed is expressed, the colonies on agar plates as well as liquid culture exhibit a bright pink color. The iGEM Team Braunschweig 2013 used this device to distinguish two different strains when cultivation in a mixed culture.

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••••• iGEM Team Braunschweig 2013

When expressed, the chromoprotein eforRed exhibits a distinct pink color. In order to avoid absorption by the chomoprotein during OD measurement of a bacterial culture, the absorption and emission spectra were measured. An optimal wavelength for spectral optical density measurement was determined to be at 520nm. iGEM Team Braunschweig 2013: Absorptionspectrum of chromoprotein eforRed in the wavelength range 400-800 nm. Absorption maximum of eforRed: 534 nm iGEM Team Braunschweig 2013: Emission spectrum of chromoprotein eforRed. The chromoprotein does show significant emission and can be detected by its fluorescence. Emission maximum of eforRed: 605 nm The optical density of cultures with cells expressing aeBlue was measured at a wavelength of 520 nm to avoid errors in measurement through absorption by the protein. iGEM Team Braunschweig 2013: Supernatant after cell disruption containing chromoproteins of eforRed and amilGFP (BBa_K1073024). Both chromoproteins fluoresce when excited by UV-light.

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