Part:BBa_K1073022:Experience

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Applications of BBa_K1073022

When eforRed is expressed, the colonies on agar plates as well as liquid culture exhibit a bright pink color. The iGEM Team Braunschweig 2013 used this device to distinguish two different strains when cultivation in a mixed culture.

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••••• iGEM Team Braunschweig 2013

When expressed, the chromoprotein eforRed exhibits a distinct pink color. In order to avoid absorption by the chomoproteins during OD measurement of a bacterial culture, the absorption and emission spectra were measured and an optimal wavelength for spectral optical density measurement was determined to be at 520nm. iGEM Team Braunschweig 2013: Absorptionspectrum of chromoprotein eforRed in the wavelength range 400-800 nm. Absorption maximum of eforRed: 534 nm iGEM Team Braunschweig 2013: Emission spectrum of chromoprotein eforRed. This chromoprotein does show significant emission and can be detected by its fluorescence. Emission maximum of eforRed: 605,4 nm According to these results the optical density for growth curves of cells expressing this protein was determined at 520 nm with respect to the chromoproteins amilGFP (BBa_K1073024) and aeBlue (BBa_K1073020) to avoid absorption by the proteins. Comparing all absorption spectra they all show an absorption minimum at 520 nm. iGEM Team Braunschweig 2013: Supernatant after cell disruption containing chromoproteins of eforRed and amilGFP (BBa_K1073024). Both chromoproteins fluoresce when excited by UV-light.

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