Composite

Part:BBa_K1065308:Design

Designed by: Caterina Marchioretti   Group: iGEM13_UNITN-Trento   (2013-09-15)
Revision as of 10:53, 28 September 2013 by Cate (Talk | contribs) (→‎Design Notes)

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J23100+YF1+FixJ+FixK2+inverter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 605
    Illegal NgoMIV site found at 677
    Illegal NgoMIV site found at 767
    Illegal NgoMIV site found at 785
    Illegal NgoMIV site found at 1297
    Illegal NgoMIV site found at 1590
    Illegal NgoMIV site found at 1684
    Illegal AgeI site found at 319
    Illegal AgeI site found at 1465
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1354
    Illegal BsaI.rc site found at 218


Design Notes

This part was built following the standard assembly protocol using BBa_K1065306 and BBa_S04617.

Source

This part has been built using:
BBa_K1065306 produces YF1 and FixJ under the control of a strong promoter of the Anderson family.
BBa_S04617 the inverter.

References

1. Andreas Möglich, Rebecca A. Ayers, Keith Moffat (2009). Design and Signaling Mechanism of Light-Regulated Histidine Kinases. Journal of Molecular Biology, 1433–1444.
2. Michael B. Elowitz & Stanislas Leibler (2000). A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338.
3.Dodd IB, Perkins AJ, Tsemitsidis D, Egan JB (2001). Octamerization of lambda CI repressor is needed for effective repression of P(RM) and efficient switching from lysogeny. Genes Dev. 15(22):3013-22.