Device

Part:BBa_K1065300:Design

Designed by: Bruno Aor   Group: iGEM13_UNITN-Trento   (2013-09-23)
Revision as of 09:05, 28 September 2013 by Aresade (Talk | contribs) (→‎Design Notes)


R0010-YF1-FixJ-PfixK2-amilGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 770
    Illegal NgoMIV site found at 842
    Illegal NgoMIV site found at 932
    Illegal NgoMIV site found at 950
    Illegal NgoMIV site found at 1462
    Illegal NgoMIV site found at 1755
    Illegal NgoMIV site found at 1849
    Illegal AgeI site found at 484
    Illegal AgeI site found at 1630
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1519
    Illegal BsaI.rc site found at 383


Design Notes

We created the part inserting R0010 in the front of K952003 device. In addition we doing a mutagenesis at the part to insert RBS before the amilGFP.

Source

R0010 derived from the registry, at the same K952003 derived from the registry

References