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Part:BBa_K1129024

Designed by: UBC iGEM 2013   Group: iGEM13_British_Columbia   (2013-09-16)
Revision as of 03:57, 28 September 2013 by AnnaMüller (Talk | contribs)

Feruloyl-CoA-Synthetase (FCS) under pTET constitutive promoter

Const+rbs+fcs+term

Feruoyl-CoA synthase is an enzyme of the vanillin pathway. It adds acetyl-CoA to ferulic acid to make ferruryl CoA the immediate precursor for vanillin.Feruoyl-CoA is under a constitutive promoter Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 104
    Illegal XhoI site found at 1214
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 91
    Illegal NgoMIV site found at 355
    Illegal NgoMIV site found at 833
    Illegal NgoMIV site found at 1088
    Illegal NgoMIV site found at 1151
    Illegal NgoMIV site found at 1168
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 863


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