Coding

Part:BBa_K1129046

Designed by: UBC iGEM 2013   Group: iGEM13_British_Columbia   (2013-09-16)
Revision as of 03:34, 28 September 2013 by AnnaMüller (Talk | contribs)

4-coumarate 3-hydroxylase under pTET constitutive promoter

Const.+rbs+4-CMH

4-coumarate 3-hydroxylase is an enzyme in the vanillin pathway. 4-coumarate 3-hydroxylase (4-CMH) hydrolyses p-coumaric acid to caffeic acid. It is the 2nd enyzme the biosynthetic pathway of vanillin from the amino acid tyrosine. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 100
    Illegal NgoMIV site found at 108
    Illegal NgoMIV site found at 858
    Illegal NgoMIV site found at 1031
    Illegal NgoMIV site found at 1433
    Illegal NgoMIV site found at 1437
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 632


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