Composite

Part:BBa_K1087006:Design

Designed by: Tong Xu   Group: iGEM13_SCU_China   (2013-09-12)
Revision as of 03:21, 28 September 2013 by Nianhui (Talk | contribs) (→‎References)

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delta activator from phiR73 phage without RBS but with B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No


Source

We ligated phiR73 transcription unit with teminator to create BBa_K1087005, then we didi PCR using BBa_K1087005 as a template to get BBa_K1087006( phiR73 activator without RBS but with B0015).

References

http://2009.igem.org/Team:Cambridge/Project/Amplification/Characterisation