Coding

Part:BBa_K1129025

Designed by: UBC iGEM 2013   Group: iGEM13_British_Columbia   (2013-09-16)
Revision as of 02:49, 28 September 2013 by Fisal (Talk | contribs)

Phenylalanine Ammonia Lyase (Enc-P) under arabinose promoter

Arab+rbs+EncP+term

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 691
    Illegal NgoMIV site found at 794
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1390
    Illegal BsaI.rc site found at 1645

Phenylalanine Ammonia Lyase (PAL) is an enzyme responsible for the conversion of the amino acid phenylalanine to trans-cinnamic acid. PAL is the first committed enzyme in the Phenylpropanoid pathway and is therefore involved in lignin degradation. PAL’s can be found in plants, fungi, and bacteria and it has a molecular mass in the range of 270-330 kDa. The deaminating action of PAL is conserved among the ammonia Lyase family that includes Histadine Ammonia Lyase, and Tyrosine Ammonia Lyase.

Useful resources

1) http://en.wikipedia.org/wiki/Phenylalanine_ammonia-lyase

2) http://www.genome.jp/kegg-bin/show_pathway?map00940

3) http://www.uniprot.org/uniprot/P25872



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