Coding

Part:BBa_K1162006

Designed by: Ryan Putman   Group: iGEM13_Utah_State   (2013-09-15)
Revision as of 02:09, 28 September 2013 by Ryanputman (Talk | contribs)

LL-37 antimicrobial peptide from Humans (Homo sapiens)

LL-37 is an antimicrobial peptide (AMP) found in humans.

Design

This part was codon optimized for E. coli using the Life Technologies GeneArt ® software program. By design, this part is Assembly Standard #23 (Silver Fusion) and contains a Methionine(atg) residue at the start of the coding region. Furthermore, this LL-37 sequence does not have any stop codons, which allows for composite BioBrick construction.

Improvement on Existing Parts (as of September 2013)

BBa_K245114 Slovenia iGEM team 2009 - lacks a start codon. Previously this part has been used Trieste iGEM team 2012 https://parts.igem.org/Part:BBa_K875009?title=Part:BBa_K875009 This part, BBa_K875009, has a BioBrick prefix and suffix of RFC 10 so cannot be used for protein fusions, such as in cases of secretion and protein purification. Also has a stop codon which limits what can be added to the C-terminal end (purification tag/secretion tag).

References

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//collections/antimicrobial
Parameters
n/aLL-37 antimicrobial peptide from Humans (Homo sapiens)