Reporter

Part:BBa_K1021020:Design

Designed by: Swati Sureka   Group: iGEM13_Cornell   (2013-09-13)
Revision as of 02:06, 28 September 2013 by S.Sureka (Talk | contribs) (→‎References)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


PT7+GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 696


Design Notes

This reporter does not contain a bacterial RBS and is therefore suited for T7-based expression in eukaryotes.


Source

The promoter originates from the T7 virus and the gene originates from the vector pHTSUB-105.

References

1. T7 promoter part page
2. GFP+LVA part page