Generator

Part:BBa_K1021033:Design

Designed by: Swati Sureka   Group: iGEM13_Cornell   (2013-09-15)
Revision as of 02:00, 28 September 2013 by S.Sureka (Talk | contribs) (→‎References)


PT7+hph


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 391
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

As this part was not designed for bacterial expression, it does not contain a bacterial RBS.


Source

The promoter comes from the T7 virus; hph comes from E. coli and was taken from the pUCATPH transformation vector.

References

[http://www.pnas.org/content/91/26/12649.full.pdf Tagged mutations at the Toxl locus of Cochliobolus heterostrophus by restriction enzyme-mediated integration]
[http://www.snapgene.com/resources/plasmid_files/yeast_plasmids/pUCATPH/ pUCATPH sequence on SnapGene]