Part:BBa_K1162004

Cg-Defh1 antimicrobial peptide from the Pacific oyster (Crassostrea gigas)

CG-Defh1 originates from immune cells in an oyster species. As a defensin AMP, CG-Defh1 contains four disulfide bonds and is most effective against gram-positive bacteria. The specific mode of action for this AMP is to inhibit peptidoglycan synthesis in the bacterial cell wall. This makes CG-Defh1 unique from many AMPs in that it does not target cell membranes. In fact, it was the first defensin from an invertebrate to exhibit antibacterial properties. Most importantly, CG-Defh1 inhibits the growth of S. aureus, a dangerous cause of hospital-aquired infections in the world.

Design

This part was codon optimized for E. coli using the Life Technologies GeneArt ® software program. By design, this part is Assembly Standard #23 (Silver Fusion) and contains a Methionine(atg) residue at the start of the coding region.

References

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]