Device

Part:BBa_K1092003:Experience

Designed by:   Group: iGEM13_Hong_Kong_CUHK   (2013-09-15)
Revision as of 01:22, 28 September 2013 by MaryZhu (Talk | contribs) (→‎Characterization of BBa_K1092003)


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Characterization of BBa_K1092003

1. Biobrick DNA length verification To check the inserted biobrick DNA length, PCR was conducted. Di.jpg

The PCR product meets the target size (936bp), the DNA sequence was further verified by DNA sequencing.

2. Time series degradation assay

Time series analysis were performed. According to a chi-square test (Rosenberg et al., 1998), the trends of hydroquinone degradation rate in E. coli BL21 transformed with catechol 1,2-dioxygenase and that in wild type E. coli BL21 were very highly statistically different (p < 0.001) (Figure 1).


Time series analysis.jpg

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UNIQ9036a306b59e8c78-partinfo-00000000-QINU UNIQ9036a306b59e8c78-partinfo-00000001-QINU