Regulatory

Part:BBa_K1225000

Designed by: James Nolan   Group: iGEM13_Purdue   (2013-09-16)
Revision as of 01:19, 28 September 2013 by CThompson (Talk | contribs)


PART DESCRIPTION:Constitutive promoter

This is a medium-strength Escherichia coli Ptrc* promoter. The asterisk denotes the lack of an operator downstream of the transcription start site, -35 to +1 of the promoter. The promoter's sequence comes from "Precise and reliable gene expression via standard transcription and translation initiation elements" by Vivek K. Mutalik and colleagues. Two inward pointing BpiI (BbsI) sites were added between the original promoter sequence and the BioBrick prefix and suffix to allow seamless assembly into the 2013 Purdue iGEM team's Bicistronic Design (BCD) Expression Operating Units, modified versions of BCDs 7, 18, 22 and 23 created by Mutalik et al. in the aforementioned work.


Contact Information


  • Author(s): James Nolan and Chris Thompson
  • Team: Purdue University 2013
  • Data Collection: Amanda Shanley, Chris Thompson, and James Nolan
  • Affiliation: Purdue University (Bindley Bioscience Center)
  • Contact: nolan6@purdue.edu
  • Related Parts: None
  • Date: 09/27/13

Standard Design Information


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


  • Chassis: E.coli
  • Strain:BL21
  • Device Name:BBa_K1225000
  • Device Type:Promoter
  • Safety Level:Risk Group 1
  • Assembly:Golden Gate Assembly
  • Protocol:Freiburg Golden Gate Protocol
  • Scars:None
  • Insertion:Plasmid
  • Vector:pSB1C3

Growth Rate Assay


BASIC INFORMATION
  • Purpose:To assess what effect, if any, our genetic parts have on the growth rate of E.coli.
  • Chassis:E.coli
  • Strain:BL21
  • Protocols:Purdue iGEM Growth Curve Protocol
  • Date:09/25/13

    • GROWTH CONDITIONS
    • Media Type:Luria Broth (LB)
    • Vessel:10mL Culture Tube
    • Volume:5mL
    • Incubation:37 C, 250 rpm

    MEASUREMENT INFORMATION
    • Data Type:Growth Curve (OD vs Time)
    • Location:Bindley Bioscience Center
    • Machine Name:N/A
    • Time Interval:30min
    • Total Time:270min

    Proof of Functionality


    BASIC INFORMATION
    • Purpose:To qualitatively and quantitatively prove that our promoter initiates transcription by placing mRFP after it.
    • Chassis:E.coli
    • Strain:BL21
    • Protocols:None
    • Date:09/26/13

      • GROWTH CONDITIONS
      • Media Type:Luria Broth (LB)
      • Vessel:10mL Culture Tube
      • Volume:5mL
      • Incubation:37 C, 250 rpm

      MEASUREMENT INFORMATION
      • Data Type:Fluorescence Intensity vs Wavelength
      • Location:Bindley Bioscience Center
      • Machine Name:N/A
      • Time Interval:30min
      • Total Time:270min


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