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Designed by: UBC iGEM 2013   Group: iGEM13_British_Columbia   (2013-08-30)
Revision as of 01:07, 28 September 2013 by AnnaMüller (Talk | contribs) (→‎Applications of BBa_K1129006)


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Applications of BBa_K1129006

The Cas9 construct was transformed into E. cloni 10G, plated on LB agar plates made with chloramphenicol (33ug/mL), and grown overnight at 37C. A single colony was picked and grown overnight in LB (with chloramphenicol) at 30C and shaking (1000 rpm). Growth was very slow for the Cas9 culture and OD was only ~0.6 in the morning. Several aliquots of 175 uL were taken and placed in separate wells of a 96-well plate. They were induced with varying concentrations of arabinose (0, 0.5%, 1%, and 2%) for three hours at 37C and 1000 rpm.


[File:UBC-Growth_curves_MOI.png|800px]

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