Coding
tetA(C)b

Part:BBa_J31006

Designed by: Sabriya Rosemond, Erin Zwack   Group: iGEM06_Davidson   (2006-07-12)
Revision as of 15:55, 28 July 2006 by Fizzle6821 (Talk | contribs)

tetracycline resistance protein TetA(C) (backwards) [cf. BBa_J31007]

This is the coding region for Tetracyline resistance in the reverse orientation. If a promoter adn RBS are placed downstream and the entire region, from promoter to stop codon is flipped the cell will express tetracycline resistance. PLEASE READ THE NOTE ABOUT THIS PART'S BIOBRICKS ON THE PART DESIGN PAGE.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1043
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 897
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 343
    Illegal NgoMIV site found at 503
    Illegal NgoMIV site found at 871
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/selectionmarker/antibioticresistance
Parameters
functionRep
ligandstetracycline
proteinTetA(C)-bkw
swissproP02981