Coding
"Eye 1"

Part:BBa_K1080000

Designed by: Macquarie University   Group: iGEM13_Macquarie_Australia   (2013-09-22)
Revision as of 00:34, 28 September 2013 by DominicLogel (Talk | contribs)

ChlI1


Magnesium chelatase subunit I - The first step in chlorophyll biosynthesis, this forms an ATP dependent hexameric ring complex and a complex with the ChlD subunit (probably a double hexameric ring complex) before acting on the protoporphyrin which is bound to the ChlH protein to insert magnesium [PMID: 11469861]. Transcript is light regulated and may be diurnal and/or circadian [PMID: 16228385]; predicted chloroplast targeting sequence amino acids 1-54 by ChloroP.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 231
    Illegal BglII site found at 887
    Illegal BglII site found at 1082
    Illegal BamHI site found at 279
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]




Amino Acid Sequence

MAATEVKAAE GRTEKELGQA RPIFPFTAIV GQDEMKLALI LNVIDPKIGG VMIMGDRGTG KSTTIRALAD LLPEMQVVAN DPFNSDPTDP ELMSEEVRNR
VKAGEQLPVS SKKIPMVDLP LGATEDRVCG TIDIEKALTE GVKAFEPGLL AKANRGILYV DEVNLLDDHL VDVLLDSAAS GWNTVEREGI SISHPARFIL
VGSGNPEEGE LRPQLLDRFG MHAQIGTVKD PRLRVQIVSQ RSTFDENPAA FRKDYEAGQM ALTQRIVDAR KLLKQGEVNY DFRVKISQIC SDLNVDGIRG
DIVTNRAAKA LAAFEGRTEV TPEDIYRVIP LCLRHRLRKD PLAEIDDGDR VREIFKQVFG ME

References and documentation are available. Please note the modified algorithm for extinction coefficient.

Number of amino acids: 362

Molecular weight: 39952.7

Theoretical pI: 5.11

Amino acid composition:
Ala (A) 31 8.6%
Arg (R) 28 7.7%
Asn (N) 12 3.3%
Asp (D) 29 8.0%
Cys (C) 3 0.8%
Gln (Q) 13 3.6%
Glu (E) 29 8.0%
Gly (G) 28 7.7%
His (H) 4 1.1%
Ile (I) 25 6.9%
Leu (L) 34 9.4%
Lys (K) 19 5.2%
Met (M) 10 2.8%
Phe (F) 12 3.3%
Pro (P) 19 5.2%
Ser (S) 14 3.9%
Thr (T) 17 4.7%
Trp (W) 1 0.3%
Tyr (Y) 4 1.1%
Val (V) 30 8.3%
Pyl (O) 0 0.0%
Sec (U) 0 0.0%

(B)   0	  0.0%
(Z)   0	  0.0%
(X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 58 Total number of positively charged residues (Arg + Lys): 47

Atomic composition:

Carbon C 1752 Hydrogen H 2857 Nitrogen N 499 Oxygen O 539 Sulfur S 13

Formula: C1752H2857N499O539S13 Total number of atoms: 5660

Extinction coefficients:

Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.

Ext. coefficient 11585 Abs 0.1% (=1 g/l) 0.290, assuming all pairs of Cys residues form cystines


Ext. coefficient 11460 Abs 0.1% (=1 g/l) 0.287, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 

                            30 hours (mammalian reticulocytes, in vitro).
                           >20 hours (yeast, in vivo).
                           >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 29.73 This classifies the protein as stable.


Aliphatic index: 96.16

Grand average of hydropathicity (GRAVY): -0.250

Source

Chlamydomonas reinhardtii

References

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Categories
Parameters
None