Regulatory

Part:BBa_K1100140:Design

Designed by: Hao-tian Guo   Group: iGEM13_Fudan   (2013-09-13)
Revision as of 23:48, 27 September 2013 by Nzy (Talk | contribs) (→‎Design Notes)

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J23100-ALeaderT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To test the performance of ALeaderT after different promoters. The plasmid map of K1100140.png

Source

J23100 is from DNA Distribution; ALeaderT is obtained via de novo gene synthesis

References

[1]Jia X, Zhang J, Sun W, et al. Riboswitch Control of Aminoglycoside Antibiotic Resistance[J]. Cell, 2013, 152(1): 68-81.