Composite

Part:BBa_K1036006

Designed by: Zhaopeng Cheng, Xiyu Wu   Group: iGEM13_XMU-China   (2013-09-09)
Revision as of 23:16, 27 September 2013 by Tina Zhang (Talk | contribs) (→‎Experimental Data)

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lux pL controlled luxR with lux pR controlled sfgfp (with LVA-tag)

Description

In this part both sfgfp and luxI genes are under the control of quorum sensing promoter, which is made up with lux pL controlled luxR with lux pR. In this case, sfgfp can express periodically according to the quorum sensing adjustment. Superfolder GFP with a LAV-tag is introduced in Part:BBa_K1036004, and quorum sensing mechanism is described in Part:BBa_K1036003.

Experimental Data

Fig.1 showed the correct result of this part construction and Fig.2 showed the distinguish of BBa_K1036005 which was without lux promoter and BBa_K1036006 which was under lux promoter control.





Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3903
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1101
    Illegal BsaI.rc site found at 2080
    Illegal BsaI.rc site found at 3183
    Illegal SapI.rc site found at 1197


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Parameters
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