Part:BBa_K1080010

YCF54

Long Descriptions here

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 108
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 270

Amino acid sequence

MAPAAASADK ATAAEYYALV CNAEWFFMDP QNESVAEQLR EKVRFFKEQN KERDFFIVPN
PKWLDAKFPE QAKQVKRPCV ALVSTDKMWI TFMKLRLDRV LKIDLKSMPA SEVLAAGEAL
PDFKPDGKWT APYARYTPGW WNVFLPNH </F>

References and documentation are available. Please note the modified algorithm for extinction coefficient.

Number of amino acids: 148

Molecular weight: 17073.7

Theoretical pI: 8.50

Amino acid composition: Ala (A) 20 13.5% Arg (R) 7 4.7% Asn (N) 6 4.1% Asp (D) 9 6.1% Cys (C) 2 1.4% Gln (Q) 5 3.4% Glu (E) 10 6.8% Gly (G) 3 2.0% His (H) 1 0.7% Ile (I) 3 2.0% Leu (L) 11 7.4% Lys (K) 14 9.5% Met (M) 5 3.4% Phe (F) 10 6.8% Pro (P) 12 8.1% Ser (S) 5 3.4% Thr (T) 5 3.4% Trp (W) 6 4.1% Tyr (Y) 4 2.7% Val (V) 10 6.8% Pyl (O) 0 0.0% Sec (U) 0 0.0%

(B)   0	  0.0%
(Z)   0	  0.0%
(X)   0	  0.0%

Total number of negatively charged residues (Asp + Glu): 19 Total number of positively charged residues (Arg + Lys): 21

Atomic composition:

Carbon C 785 Hydrogen H 1190 Nitrogen N 202 Oxygen O 212 Sulfur S 7

Formula: C785H1190N202O212S7 Total number of atoms: 2396

Extinction coefficients:

Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.

Ext. coefficient 39085 Abs 0.1% (=1 g/l) 2.289, assuming all pairs of Cys residues form cystines

Ext. coefficient 38960 Abs 0.1% (=1 g/l) 2.282, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is:

                            30 hours (mammalian reticulocytes, in vitro).
                           >20 hours (yeast, in vivo).
                           >10 hours (Escherichia coli, in vivo).

Instability index:

The instability index (II) is computed to be 35.64 This classifies the protein as stable.

Aliphatic index: 70.00

Grand average of hydropathicity (GRAVY): -0.385

Source

Chlamydomonas reinhardtii