Generator

Part:BBa_K1124011:Design

Designed by: Yuta Otsuka   Group: iGEM13_UT-Tokyo   (2013-09-11)
Revision as of 21:41, 27 September 2013 by Minakuchi.T (Talk | contribs) (→‎Source)

hpaBC (+RBS(natural))


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 97
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1877


Design Notes

This part was cloned from E. coli BL21(DE3) genome by PCR. We added restriction sites on both ends of the genomic sequence.

Source

E. coli BL21(DE3)

Links to NCBI gene detabase

http://www.ncbi.nlm.nih.gov/gene/8183130 (hpaB)

http://www.ncbi.nlm.nih.gov/gene/8183129 (hpaC)

References