DNA

Part:BBa_K1054008

Designed by: Jiasheng Wang   Group: iGEM13_ZJU-China   (2013-09-10)
Revision as of 21:32, 27 September 2013 by Stargazin (Talk | contribs)

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pLAC promotor,atrazine-inducible Riboswitch, GFP+Terminator

This is the part used to standardize the atrazine Riboswitch.

The protocol is as follows: 1. Cultivate the BL21 containing BBa_ in LB at 37℃180rpm until OD600 reaches 0.5.

2. Induce the bacteria with 1mM IPTG (final concentration), and cultivate them at 37℃180rpm for 4hrs.

3. Dilute the suspension culture with LB to 0.1OD600 . Theophylline is added at concentration ranging from 1.00E-06 mol/L to1.00E-01 mol/L.

4. Measure the RFU ( ex488/em525) and OD600 synchronously.

5. Data Analysis

500px-ZJU-Standardization 2.1.jpg

Graphic Model about how RFU/OD changes at different time and different concentration of Atrzine.

500px-ZJU-Standardization 2.2.jpg

Graphic Model about how GFP Change Rate changes at different time and different concentration of Atrzine.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 217
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 937


[edit]
Categories
//rna/riboswitch
Parameters
n/apLAC promotor,atrazine-inducible Riboswitch, GFP+Terminator