Part:BBa_K1100011

B0015 terminator efficiency estimation device (with Csy4 system)

In this device, we test the terminator efficiency in the consideration that the terminator is influenced by the downstream sequence. The csy4 cleavage site is inserted just after the terminator(BBa_B0015).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 448
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 164
  

Histogram of the Flow cytometer measurement