Part:BBa_K1166005
TAT-Apoptin
Codes for the T7 expression cassette for Apoptin composed of a N-terminal TAT translocation peptide, followed by a linker (GGGGS), a histidine tag (6x) and by the same linker fused to Apoptin. Apoptin, also called VP3, is a protein from the Chicken Anemia Virus (CAV) known to cause p53-independent apoptosis in more than 70 human cancer cell lines while leaving normal cells unharmed (Backendorf, et al., 2008).
Apoptin contains a bipartite nuclear localization signal (NLS1: aa 82-88 and NLS2: aa 111-121) and a nuclear export signal (NES) (aa 97-105) (Heckl, et al., 2008). In cancer cells, Apoptin localizes to the nucleus while in normal cells it's found in the cytoplasm; it is thought that the bipartite NLS is activated by phosphorylation at Threonine 108 which only happens in cancer cells (Rohn, et al., 2002).
References
Backendorf C, Visser AE, de Boer AG, Zimmerman R, Visser M, Voskamp P, Zhang YH, Noteborn M. (2008). Apoptin: therapeutic potential of an early sensor of carcinogenic transformation. Annu Rev Pharmacol Toxicol. 48:143-69.
Heckl S, Regenbogen M, Sturzu A, Gharabaghi A, Feil G, Beck A, Echner H, Nagele T. (2008). Value of apoptin's 40-amino-acid C-terminal fragment for the differentiation between human tumor and non-tumor cells.Apoptosis. 13(4):495-508
Rohn JL, Zhang YH, Aalbers RI, Otto N, Den Hertog J, Henriquez NV, Van De Velde CJ, Kuppen PJ, Mumberg D, Donner P, Noteborn MH. (2002). A tumor-specific kinase activity regulates the viral death protein Apoptin. J Biol Chem. 277(52):50820-7
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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