Regulatory

Part:BBa_K1051304

Designed by: Kequan Lin   Group: iGEM13_Shenzhen_BGIC_ATCG   (2013-06-30)
Revision as of 16:59, 27 September 2013 by WHUyoyo (Talk | contribs)

cln3 promoter (M phase to G1 phase)

Purpose

work to activates Cdc28p kinase to promote the G1 to S phase transition

Principle

CLn3 is a promoter which promotes transciption in M of the yeast cell cycle .If you want to express your proteins in M of the yeast cell cycle ,you can add the sequence which is actived by cln3 before your gene sequence ,then put them in a yeast plasmid .After transformation ,you can get the protein you want from the metabolic product of the Bacterial you use for transformation .it plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis; acetly-CoA induces CLN3 transcription in response to nutrient repletion to promote cell-cycle entry.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 661
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/a cln3 promoter (M phase to G1 phase)