DNA

Part:BBa_K1051200

Designed by: wanling ZHOU   Group: iGEM13_Shenzhen_BGIC_ATCG   (2013-06-30)
Revision as of 16:23, 27 September 2013 by Zyllei (Talk | contribs)

Ubiquitin

Purpose

Works for the degradation of fluorescent proteins we used.

Principle

Ubiquitin,a small regulatory protein that has been found in almost all tissues of eukaryotic organisms,Ubiquitination is a post translational modification where ubuiquitin is attached to a substrate protein.It is carried out in three main steps; activation, conjugation and ligation, performed by ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin ligases (E3s).

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 233
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 25
    Illegal AgeI site found at 253
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aUbiquitin