Signalling

Part:BBa_K1024004:Design

Designed by: Sun Xiaochen   Group: iGEM13_Tsinghua   (2013-09-09)
Revision as of 16:17, 27 September 2013 by Fanxiao0606 (Talk | contribs) (→‎References)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Sensor yeast (inducible TetR+VP16)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2555
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1580
    Illegal BamHI site found at 2546
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2555
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2555
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 506
    Illegal BsaI site found at 893
    Illegal BsaI site found at 1280
    Illegal BsaI.rc site found at 547
    Illegal BsaI.rc site found at 934
    Illegal BsaI.rc site found at 1321


Design Notes

Design: The part was the sensor of the pathogen detector. LuxR was constitutively expressed. When sensing AHL, LuxR will bind to the Lux promoter and thus activated the downstream TetR with VP16. Mated with reporter yeast (BBa_K1024005), the TetR with VP16 will bind to the Tet operator and activated the downstream gene.

Source

S. cerevisiae & Registry

References


[1]Fuqua W C, Winans S C, Greenberg E P. Quorum sensing in bacteria: the LuxR-LuxI family of cell density-responsive transcriptional regulators[J]. Journal of bacteriology, 1994, 176(2): 269.
[2]G, Garí E, Piedrafita L, et al. An activator/repressor dual system allows tight tetracycline-regulated gene expression in budding yeast[J]. Nucleic acids research, 1998, 26(4): 942-947.