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Part:BBa_K1149050:Design

Designed by: Margarita Kopniczky   Group: iGEM13_Imperial_College   (2013-09-24)
Revision as of 13:38, 27 September 2013 by Margarita K (Talk | contribs) (→‎Source)

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Bdh2 (intracellular)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 181
    Illegal AgeI site found at 1126
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We removed secretion tag to make intracellular. While doing this, we linearised the plasmid with PCR to exclude the secretion tag in construct BBa_K1149013. Then, we did blunt ended ligation to circularize the plasmid again. During the ligation, 3 nucleatodes disappeared fron the scar between the RBS and the start of the bdh2 gene. Therefore the scar is "TAC" istead of the regular "tactag".

Source

team: Imperial College 2013 iGEM Team

organism: Ralstonia pickettii

References

Characterization of Two 3-Hydroxybutyrate Dehydrogenases in Poly(3-Hydroxybutyrate)-Degradable Bacterium, Ralstonia pickettii T1 , Masahiko Takanashi and Terumi Saito, 2006