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Part:BBa_K1149050:Design

Designed by: Margarita Kopniczky   Group: iGEM13_Imperial_College   (2013-09-24)
Revision as of 13:36, 27 September 2013 by Margarita K (Talk | contribs) (→‎References)


Bdh2 (intracellular)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 181
    Illegal AgeI site found at 1126
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We removed secretion tag to make intracellular. While doing this, we linearised the plasmid with PCR to exclude the secretion tag in construct BBa_K1149013. Then, we did blunt ended ligation to circularize the plasmid again. During the ligation, 3 nucleatodes disappeared fron the scar between the RBS and the start of the bdh2 gene. Therefore the scar is "TAC" istead of the regular "tactag".

Source

Imperial iGEM 2013

References

Characterization of Two 3-Hydroxybutyrate Dehydrogenases in Poly(3-Hydroxybutyrate)-Degradable Bacterium, Ralstonia pickettii T1 , Masahiko Takanashi and Terumi Saito, 2006