Composite

Part:BBa_K1074006

Designed by: Changlong Zhao   Group: iGEM13_USTC_CHINA   (2013-09-09)
Revision as of 13:22, 27 September 2013 by Nocturne (Talk | contribs)

Pgrac+RBS+SamyQ+TD1+GFP

This is the main circuit of our project to allow high expression of target protein(antigen,adjuvant).GFP can be substituted by various proteins via a modular PCR or standard cut/ligation method.

Usage and Biology

References

SUN Zheng, WEN Long-ping.Transdermal delivery of fusion green fluorescent protein mediated by covalently associated TD1 peptide. OURNAL OF UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA.2009.Vol.39,No.4

1.张灏,夏雨,傅晓燕等.耐热β2半乳糖苷酶基因bgaB 在枯草芽孢杆菌中的整合表达.Journal of Wuxi University of Light Industry 第22卷第6期.2003年11月. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 984


[edit]
Categories
//chassis/prokaryote/bsubtilis
Parameters
None