Coding
ndh-lva

Part:BBa_K1036001:Design

Designed by: Shengquan Zeng, Zhaopeng Cheng   Group: iGEM13_XMU-China   (2013-08-27)
Revision as of 13:05, 27 September 2013 by Tina Zhang (Talk | contribs) (→‎Design Notes)

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the ndh gene coding for respiratory NADH dehydrogenase II


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Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1360
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The ndh gene was amplifed from the genome DNA of E. coli strain BL21(DE3) via PCR. Then RBS(BBa_B0034), LVA-tag and terminator(BBa_B0015) were fused with ndh via fusion PCR becoming RBS-ndh-LVA-TT marked as ndh. The DH5α colony carried ndh gene was sequenced after TA-cloning.

Source

The ndh gene was amplifed from the genome DNA of E.coli strain BL21 via PCR.

References

Messner, K. R. & Imlay, J. A. The identification of primary sites of superoxide and hydrogen peroxide formation in the aerobic respiratory chain and sulfite reductase complex of Escherichia coli. J. Biol. Chem. 274, 10119-10128 (1999)