Regulatory

Part:BBa_K1111001:Design

Designed by: Luisa Spisak   Group: iGEM13_EPF_Lausanne   (2013-09-18)
Revision as of 12:45, 27 September 2013 by GigiGoesGugu (Talk | contribs) (→‎References)


Hya promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 184
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 184
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 184
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 184
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 184
    Illegal AgeI site found at 26
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

took 500bp upstream from starting codon


Source

e.coli, mg1655

References

Journal of Bacteriology
Response of hya Expression to External pH in Escherichia coli
Paul W. King and Alan E. Przybyla
J.Bacteriol. 1990

Journal of Bacteriology
Mutational analysis and charecterization of the Escherichia coli hya operin, which encodes [NiFe] hydrogenase1
N K Menon, J Robbins, J C Wendt, K T Shanmuagam and A E Przybyla
J. Bacteriol 1991