Part:BBa_K1228006

25aa with T1 terminator and Constitutive promoter veg

This generator contains BBa_K541503(https://parts.igem.org/Part:BBa_K541503) and BBa_K1228005(https://parts.igem.org/Part:BBa_K1228005). The generator can secrete 25 aminoacid peptide that is a part of Lactoferrin in B.subtilis. It has a broad anti-bacterial range and strong anti-bacterial activity.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Experiment and Results

1. Inoculate recombinant strains into 20ml LB.Cultivate at 37°C overnight.

2. Add 200ul indicator inoculum (Micrococcus Lysodeikticus or Escherichia Coli) untill heated LB solid medium (100ml) cool to about 50°C.

3. Use puncher make several hole on the LB plate.Add 80ul recombinant strains inoculum into the hole.Cultivate at 37°C overnight.

4. Observe whether LB plate will appear inhibition zone.

Reference

1.BellamyW,TakaseM,Yamauchi K,et al. Identification of the bactericidal domainof lactoferrin[J]. BiochimicaetBiophysicaActa,1992,1121( 1/2):130-136.

2.EliassenLT,Berge G,SveinbjornssonB,et al. Evidenceforadirect antitumormechanismof actionof Bovinel actoferricin[J].AnticancerResearch,2002,22(5):2703－2710.

3.Wakabayashi H,TakaseM,TomitaM. Lactoferricin derived from milk protein lactoferrin[J]. Current Pharmaceutical Design,2003,9(16):1277－1287.